Cell line/type | JPCs (jaw periosteal cells) |
---|---|
Species | Human |
Animal free | Yes |
Product | MesenCult-XF Alexander et al. 2013 |
Alexander, D., Rieger, M., Klein, C., Ardjomandi, N., & Reinert, S. (2013). Selection of osteoprogenitors from the jaw periosteum by a specific animal-free culture medium. PloS one, 8(12), e81674. The aim of the present study is to compare and analyze the phenotype of osteoprogenitors from the jaw periosteum under normal FCS-containing and animal-free culture conditions. Therefore, we analyzed the proliferation rates of MesenCult-XF medium (MC-) in comparison to DMEM-cultured JPCs. Whereas jaw periosteal cells (JPCs) show relatively slow proliferation rates and a fibroblastoid shape under DMEM culture conditions, MC-cultured JPCs diminished their cell size significantly and proliferated rapidly. By live-monitoring measurements of adhesion and proliferation, the authors made an interesting observation: whereas the proliferation of the MSCA-1(+) subpopulation and the unseparated cell fraction were favored by the animal-free culture medium, the proliferation of the MSCA-1(-) subpopulation seemed to be repressed under these conditions. The alkaline phosphatase expression pattern showed similar results under both culture conditions. Comparison of the mineralization capacity revealed an earlier formation of calcium-phosphate precipitates under MC culture conditions; however, the mineralization capacity of the DMEM-cultured cells seemed to be higher. The authors conclude that the tested animal-free medium is suitable for the in vitro expansion and even for the specific selection of osteoprogenitor cells derived from the jaw periosteum. MesenCult-XF medium is a commercial product. The precise formulation is proprietary. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3857222/pdf/pone.0081674.pdf |
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Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |
Contains phenol red > No | No |
Antibiotics free > Yes | Yes |