Cell line/type | At-MSCs (adipose tissue derived mesenchymal stem cells) |
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Species | Human |
Animal free | Yes |
Product | IBMTstem-medium (cryopreservation) |
Zeisberger, S. M., Schulz, J. C., Mairhofer, M., Ponsaerts, P., Wouters, G., Doerr, D., ... & Zisch, A. H. (2011). Biological and physicochemical characterization of a serum-and xeno-free chemically defined cryopreservation procedure for adult human progenitor cells. Cell transplantation, 20(8), 1241-1257. In this study, the EU FP6-funded CRYSTAL (CRYo-banking of Stem cells for human Therapeutic AppLication) consortium aimed to validate novel Standard Operating Procedures to perform and validate xeno-free and chemically defined cryopreservation of human progenitor cells and to reduce the amount of the potentially toxic cryoprotectant additive DMSO. To achieve this goal, three human adult progenitor and stem cell populations—umbilical cord blood (UCB)-derived erythroid cells (UCB-ECs), UCB-derived endothelial colony forming cells (UCB-ECFCs), and adipose tissue (AT)-derived mesenchymal stromal cells (AT-MSCs)—were cryopreserved in chemically defined medium supplemented with 10% or 5% DMSO. Only three media, IBMTPLUS, IBMTSTEM filled the criterion of being free of xenogenic compounds. The results demonstrate the feasibility and robustness of standardized xeno-free cryopreservation of different human progenitor cells and encourage their use even more in the field of tissue-engineering and regenerative medicine. IBMT-medium, used in this study, is a commercially available medium. The precise formulation is proprietary. http://journals.sagepub.com/doi/abs/10.3727/096368910X547426?url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&rfr_dat=cr_pub%3Dpubmed& |
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Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |