Cell line/type | At-MSCs (adipose tissue derived mesenchymal stem cells) |
---|---|
Species | Human |
Animal free | Yes |
Product | S/D-PL |
Shih, D. T. B., Chen, J. C., Chen, W. Y., Kuo, Y. P., Su, C. Y., & Burnouf, T. (2011). Expansion of adipose tissue mesenchymal stromal progenitors in serumāfree medium supplemented with virally inactivated allogeneic human platelet lysate. Transfusion, 51(4), 770-778. In this study, platelet concentrates were treated by S/D (1% tri-n-butyl phosphate and 1% Triton X-45), extracted by oil, purified by C18 hydrophobic interaction chromatography, and sterile filtered. S/D-PL was compared to FBS as a medium supplement (10% vol/vol) for isolating, maintaining, and expanding adipose tissue-derived MSCs (AT-MSCs). AT-MSCs had a typical spindle morphology and proliferated in S/D-PL at least as well as in FBS. Immunophenotype at Passage 7 was characteristic of MSCs and similar for both culture conditions. Differentiation capacity into the three lineages was maintained and chondrogenesis was enhanced by S/D-PL. In a 120 human cytokine antibody array analysis, 73 cytokines were detected in S/D-PL, including 22 with a concentration higher than in FBS. S/D-PL is an alternative to FBS for AT-MSC maintenance and expansion, does not compromise the differentiation capacity nor the immunophenotype, and may accelerate chondrogenesis. In Fig. 1. is the preparation process of the S/D-PL listed. https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1537-2995.2010.02915.x |
|
Source | Literature - own formulation |
Chemically defined > No | No |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |