Cell line/type | At-MSCs (adipose tissue derived mesenchymal stem cells) |
---|---|
Species | Human |
Animal free | No |
Product | MSC-T4 Sato et al. 2015 |
Sato, K., Itoh, T., Kato, T., Kitamura, Y., Kaul, S. C., Wadhwa, R., ... & Ohneda, O. (2015). Serum-free isolation and culture system to enhance the proliferation and bone regeneration of adipose tissue-derived mesenchymal stem cells. In Vitro Cellular & Developmental Biology-Animal, 51(5), 515-529. In this study, the authors formulated a completely serum-free culture medium (SFM) that could be used not only for the expansion of adipose tissue-derived mesenchymal stem cells (AT-MSCs) but also for initial isolation. It was demonstrated that the AT-MSCs isolated and cultured in serum-free medium (AT-MSCs/SFM) possess high proliferation capacity and differentiation potency to osteoblast, adipocyte, and chondrocyte lineages in vitro. In in vivo bone fraction model analysis, AT-MSCs/SFM showed higher bone repair potency and quality of the regenerated bone than the cells cultured in serum-containing medium (AT-MSCs/SCM). The cells were cultured in MSC-T4 serum-free culture medium (Cell Science Technology Institute, CSTI, Sendai, Japan) for AT-MSCs, which contains bFGF, PDGFBB, TGF-β1 and bovine serum albumin in modified αMEM. The precise formulation is proprietary.
https://link.springer.com/content/pdf/10.1007%2Fs11626-014-9860-0.pdf |
|
Source | Literature - modified commercial product |
Chemically defined > Unspecified | Unspecified |