Cell line/type | hESC/hiPSCs-derived CMs (cardiomyocytes) |
---|---|
Species | Human |
Animal free | Yes |
Product | CDM3 |
Burridge, P. W., Matsa, E., Shukla, P., Lin, Z. C., Churko, J. M., Ebert, A. D., ... & Plews, J. R. (2014). Chemically defined generation of human cardiomyocytes. Nature methods, 11(8), 855. In this study, hiPSCs were derived under chemically defined conditions on synthetic matrices. A systematically optimized cardiac differentiation strategy was developed using a chemically defined medium consisting of just three components: the basal medium RPMI 1640, l-ascorbic acid 2-phosphate and rice-derived recombinant human albumin, termed ‘CDM3’ medium. Along with small molecule-based induction of differentiation, this protocol produced contractile sheets of up to 95% TNNT2+ cardiomyocytes at a yield of up to 100 cardiomyocytes for every input pluripotent cell and was effective in 11 hiPSC lines tested. This chemically defined platform for cardiac specification of hiPSCs will allow the elucidation of cardiomyocyte macromolecular and metabolic requirements and will provide a minimal system for the study of maturation and subtype specification. Efficient differentiation with CDM3 medium was achieved over a broader range (0.8 × 104 to 1.4 × 104 cells/cm2) of initial seeding densities than those for RPMI + B27 − ins medium (1.2 × 104 to 1.4 × 104 cells/cm2). In summary, the chemically defined differentiation medium CDM3 provides a reproducible, scalable method for deriving cardiomyocytes from hiPSCs. The formulation of CDM3 is described in the results, under the heading; Optimization of a chemically defined medium, and in the Online Methods, under the heading: Cardiac differentiation of hiPSCs. https://www.nature.com/articles/nmeth.2999.pdf |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |