Lam, A. T. L., Chen, A. K. L., Li, J., Birch, W. R., Reuveny, S., & Oh, S. K. W. (2014). Conjoint propagation and differentiation of human embryonic stem cells to cardiomyocytes in a defined microcarrier spinner culture. Stem cell research & therapy, 5(5), 110.
DOI: 10.1186/scrt498.
In this study, production of hESC-derived CMs was initially established in monolayer cultures. This control condition was compared against hESC expansion on laminin-coated MC with cationic surface charge, in a stirred serum-free defined culture using RPMI/B27 medium. Following expansion, the hESC/MC aggregates were placed in a CM differentiation medium, using Wnt signalling modulators in four different culture conditions. This process eliminated the need for manual colony cutting. The final optimized protocol was tested in stirred spinner flasks, combining expansion and differentiation on the same MC, with only media changes during the culture process.
The composition of RPMI/B27 medium, referred to as differentiation medium, is listed in the Materials and Methods, under the heading; Culture regime 1: monolayer.
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