Cell line/type | hPSCs (human pluripotent stem cells) |
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Species | Human |
Animal free | Unspecified |
Product | NutriStem® V9 XF Medium |
NutriStem® V9 XF Medium is a defined, xeno-free, serum-free medium designed to support the growth and expansion of human pluripotent stem cells (hPSC), using vitronectin and enzyme-free passage using EDTA. It contains only the essential components required for long-term maintenance of hES and hiPS cells. NutriStem® V9 XF medium shows superior proliferation rates during long-term cultures, while maintaining the pluripotency of the cells. Features
Sample DataLong-term ExpansionNucleocounts performed on equal volume of cell suspension Chemometech, (count of aggregated cells) during long-term expansion of H1 cultured in NutriStem® V9 XF medium and E8 under a weekend-free feeding regime using 0.5μg/cm2 Vitronectin ACF. NutriStem® V9 XF medium shows superior proliferation rate in long-term culture (over 20 passages)
Cell morphologyPhase contrast images (x100) of H1 hESC culture maintained in NutriStem® V9 XF medium under a weekendfree feeding regime, using 0.5μg/cm2 Vitronectin ACF. Representative images from culture at P6, P16 and P20. Undifferentiated colonies maintained during long-term culture of H1 hESC
Immunofluorescence stainingImmunofluorescence analysis of human pluripotent markers of H1 hPSC expanded in NutriStem® V9 XF medium under a weekend-free feeding regime using Vitronectin ACF. Cells from P8 were fixated and stained for the classic pluripotent surface markers: TRA 1-60 (Alexa Fluor) (green) and nuclear conjugated markers: Nanog-RRX, counterstained with DAPI (blue). Scale bar 200μm. Cells cultured in NutriStem® V9 XF Medium on vitronectin express high levels of pluripotent markers
Flow cytometric quantificationImmunophenotyping analysis for human pluripotent markers of H1 hESC culture maintained in NutriStem® V9 XF medium under a weekend-free feeding regime using 0.5μg/cm2 Vitronectin ACF at P8 and P17. Data presented as % expression from gated viable cells. High expression of pluripotent stem cell markers in cells cultured in NutriStem® V9 XF medium on vitronectin https://www.bioind.com/nutristem-v9/ |
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Source | Biological Industries |
Contains phenol red | Yes |