Cell line/type | OMECs (oral mucosal epithelial cells) |
---|---|
Species | Human |
Animal free | Yes |
Product | CnT-24 Ilmarinen et al. 2013 |
Ilmarinen, T., Laine, J., Juuti‐Uusitalo, K., Numminen, J., Seppänen‐Suuronen, R., Uusitalo, H., & Skottman, H. (2013). Towards a defined, serum‐and feeder‐free culture of stratified human oral mucosal epithelium for ocular surface reconstruction. Acta ophthalmologica, 91(8), 744-750. In this study, the formation of stratified cell sheets from human oral mucosal epithelial cells under serum-free culture environment both in the absence and presence of fibroblast-conditioned culture medium and elevated epidermal growth factor (EGF) concentration was examined. In this culture method, the authors have been able to produce stratified cell sheets successfully without serum, conditioning of the medium or increased EGF concentration. A novel protocol to produce tight multi-layered epithelium with proliferative potential, which can be easily adapted for cultivated oral mucosal epithelial transplantation was provided. CnT-24 is a commercial product. The precise formulation is proprietary. https://link.springer.com/content/pdf/10.1007%2F10_2012_134.pdf |
|
Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |