Cell line/type | hESC/hPSC-derived RPE (retinal pigment epithelial cells) |
---|---|
Species | Human |
Animal free | Unspecified |
Product | SF-RPEM |
Lidgerwood, G. E., Lim, S. Y., Crombie, D. E., Ali, R., Gill, K. P., Hernández, D., ... & Liang, H. H. (2016). Defined medium conditions for the induction and expansion of human pluripotent stem cell-derived retinal pigment epithelium. Stem Cell Reviews and Reports, 12(2), 179-188. In this study, the authors assessed the differentiation of hPSCs towards retinal pigment epithelium (RPE) in the RPE-enrichment basal medium (RPEM). The combination of growth factors of RPEM medium enables a reproducible and simplified one-step method to generate and propagate RPE cells from hPSCs and is adaptable to feeder-free and serum-free conditions. This protocol also results in faster differentiation of hPSCs into RPE cells compared to timeframe reported in conventional spontaneous differentiation-based methods. The authors demonstrate that a combination of Noggin, Dickkopf-1, Insulin Growth Factor 1 and basic Fibroblast Growth Factor, promotes the differentiation of human pluripotent stem cells into RPE cells. The formulation of serum-free RPEM is listed in the Materials and Methods, under the heading; RPE Cell Differentiation https://link.springer.com/content/pdf/10.1007%2Fs12015-015-9636-2.pdf |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Antibiotics free > No | No |
Contains phenol red > Yes | Yes |