Cell line/type | hESCs H1 |
---|---|
Species | Human |
Animal free | No |
Product | KSR-based conditioned medium |
Yang, D., Chen, S., Gao, C., Liu, X., Zhou, Y., Liu, P., & Cai, J. (2016). Chemically defined serum-free conditions for cartilage regeneration from human embryonic stem cells. Life sciences, 164, 9-14. The aim of this study was to improve a method that induce cartilage differentiation of human embryoid stem cells (hESCs) in vitro, and test the effect of in vivo environments on the further maturation of hESCs derived cells. The authors were able to obtain chondrocyte/cartilage from hESCs using serum-free KSR-based conditioned medium. The results of this study provide an absolute serum-free and efficient approach for the generation of hESC-derived chondrocytes, and cells will become further maturation in vivo. This study showed that human ECSs are capable of differentiation into chondrocyte/cartilage in vitro using a KSR-based absolute serum-free protocol, and further mature in vivo. The process of hESCs differentiation was a two-stage procedure. The protocol and media formulations are listed in the Materials and Methods, under the heading; Chondrogenic differentiation. https://ac.els-cdn.com/S0024320516305501/1-s2.0-S0024320516305501-main.pdf?_tid=924533c0-480c-471b-86a9-2ab1b66591b4&acdnat=1530019944_fa455526526c4d21c1632e3a6381107a |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |