Cell line/type | Corneal tissue |
---|---|
Species | Rabbit |
Animal free | Yes |
Product | sfSHEMSB / sfSHEMA83 |
Zamudio, A., Wang, Z., Chung, S. H., & Wolosin, J. M. (2016). Inhibition of TGFβ cell signaling for limbal explant culture in serumless, defined xeno-free conditions. Experimental eye research, 145, 48-57. In this study, the authors examined the effect of TGFbeta is a cytostatic effector for epithelial proliferation. Limbal biopsies were set on explant culture in DMEM/F12 with 5 ng/ml EGF and cholera toxin (ChT), ITS, and 5% FBS, henceforth SHEM or a) SHEMSB=SHEM plus SB431542 an inhibitor of TGFbeta signaling; b) sfSHEM = SHEM with FBS replaced by 0.05% Albumax II; and c) sfSHEMSB and sfSHEMA83 = sfSHEM plus, respectively, SB431542 or A-83-01, another TGFbeta inhibitor. The authors describe that their experiments show that neither (bovine-derived) Albumax II and/or (bacterial) ChT are essential for explant outgrowth in serum free medium, at least for the initial two week explant culture. shSHEMSB or sfSHEMA83 The formulation of sfSHEMSB and sfSHEMA83 media is listed in the Materials and Methods, under the heading; Tissue procurement and cell culture procedures. https://ac.els-cdn.com/S0014483515300592/1-s2.0-S0014483515300592-main.pdf?_tid=6798429b-31bc-4cf1-9210-0e2a43a5b210&acdnat=1530019919_d2b96e08d1410f9d9d5f1aaa72f517e7 |
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Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > No | No |
Antibiotics free > Yes | Yes |