Cell line/type | hPSC-HLPCs (Human pluripotent stem cell-hepatic lineage progenitor cells) |
---|---|
Species | Human |
Animal free | No |
Product | HepSCF |
Fukuda, T., Takayama, K., Hirata, M., Liu, Y. J., Yanagihara, K., Suga, M., ... & Furue, M. K. (2017). Isolation and expansion of human pluripotent stem cell-derived hepatic progenitor cells by growth factor defined serum-free culture conditions. Experimental cell research, 352(2), 333-345. In this study, the authors have developed growth factor defined serum-free culture conditions without feeder cells for isolating hPSCHPCs, proliferating HPC-HBCs, and promoting differentiation of HPCHBCs into HPC-HCs. Because all the components of culture medium are defined, the culture conditions can constantly reproduce HPC-HCs. The authors have confirmed that the culture condition for the proliferation of HPCHBCs is capable of maintaining HPC-HBCs for at least 6 months. hPSC-HLPCs were seeded on a plastic dish coated with bovine fibronectin (Sigma-Aldrich) at 2 μg/cm2 in a serum-free medium for culturing HPCs, designated as HepSCF medium. HepSCF medium consists of HepSCF basal medium supplemented with 5 factors. The precise formulation of HepSCF medium is listed in the supplementary information, Table S1. https://ac.els-cdn.com/S0014482717300757/1-s2.0-S0014482717300757-main.pdf?_tid=584d7d3e-8610-4e6e-9e61-9b0c97998542&acdnat=1530019882_96336082959f6c5377e6a1bf8facce4a |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |