Cell line/type | CHO (Chinese Hamster Ovary) DG44 |
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Species | Hamster |
Animal free | Yes |
Product | Basal SFM + hydrolysates Kim et al. 2011 |
Kim, J. Y., Kim, Y. G., Han, Y. K., Choi, H. S., Kim, Y. H., & Lee, G. M. (2011). Proteomic understanding of intracellular responses of recombinant Chinese hamster ovary cells cultivated in serum-free medium supplemented with hydrolysates. Applied microbiology and biotechnology, 89(6), 1917-1928. In this study, a proteomic approach identified differentially expressed proteins in rCHO cells in SFM supplemented with various ratios of hydrolysate mixtures. The identified proteins can provide insights into the effect of hydrolysates on intracellular events and candidates for cell engineering to maximize cell growth and/or protein production in rCHO cells. SFM#S1 yielded the highest specific growth rate, and SFM#S2 the highest specific antibody productivity (q(Ab,). The comparative proteomic analysis with basal SFM without hydrolysates revealed that the addition of hydrolysate mixtures significantly altered the profiles of CHO proteome. In SFM#S1, the expression of metabolism-related proteins, cytoskeleton-associated proteins, and proliferation-related proteins was up-regulated. On the other hand, the expression of anti-proliferative proteins and pro-apoptotic protein was down-regulated. In SFM#S2, the expression of various chaperone proteins and proliferation-linked proteins was altered. 2D-Western blot analysis of differentially expressed proteins confirmed the proteomic results. Taken together, identification of differentially expressed proteins in CHO cells by a proteomic approach can provide insights into understanding the effect of hydrolysates on intracellular events and clues to find candidate genes for cell engineering to maximize the protein production in rCHO cells. Matrix of ten mixtures of hydrolysates using a simplex lattice design and its effect on μ and qAb2 are listed in Table 1. The composition of the basal SFM is listed in a previous article, Kim 2009 in Table 1. |
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Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > No | No |
Antibiotics free > Yes | Yes |