Cell line/type | CHO (Chinese Hamster Ovary) DG44 |
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Species | Hamster |
Animal free | Yes |
Product | CD OptiCHO Qi et al. 2016 |
Qi, Y., Xu, Y., Pan, Y., Li, S., Li, B., Pan, M., ... & Li, Y. (2016). Overexpression and purification of HSV‐2 glycoprotein D in suspension CHO cells with serum‐free medium and immunogenicity analysis. Biotechnology and applied biochemistry, 63(3), 312-319. In this study, a stable, secretory, and high-yield gD2-expression cell line and an easy and efficient gD2-purification process was established, which lays the foundation for preparation of large amount of gD2 that is essential for HSV-2 subunit vaccine development. The recombinant plasmid pMD902-gD was stably transfected into CHO-DG44 cells, and cell lines with high levels of expression of gD2 were established. The yield of the purified gD2 was 57 mg/L of serum-free culture medium, and its purity was determined to be about 95% by HPLC analysis. The cells were cultured in CD OptiCHO supplemented with different components as specified in the Materials and Methods, under the heading 2.3. Screening of stably transfected cell lines and MTX amplification. https://iubmb.onlinelibrary.wiley.com/doi/abs/10.1002/bab.1386 |
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Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |
Contains phenol red > No | No |
Antibiotics free > Yes | Yes |