Cell line/type | CHO (Chinese Hamster Ovary) DG44 |
---|---|
Species | Hamster |
Animal free | Yes |
Product | serum-free CD DG44 medium |
Jazayeri, S. H., Amiri-Yekta, A., Gourabi, H., Emami, B. A., Halfinezhad, Z., Abolghasemi, S., ... & Khorramizadeh, M. R. (2017). Comparative assessment on the expression level of recombinant human follicle-stimulating hormone (FSH) in serum-containing versus protein-free culture media. Molecular biotechnology, 59(11-12), 490-498. In this study, the authors assessed the expression level of recombinant human follicle-stimulating hormone (rhFSH) in adherent and suspension Chinese hamster ovary (CHO) cell lines by cultivation in serum-containing and chemically defined, protein-free media. The authors comparatively assessed epithelial adherent CHO/dhfr- and suspension CHO DG44 cells to obtain best-yield, MTX-treated clone for recombinant human FSH in serum-containing versus serum-free media. Collectively, adherent CHO/dhfr- in serum-containing showed considerably lower secretion rates as compared to suspension DG44 cells in protein-free media and rhFSH could be more economically produced by DG44 cell system. Serum-free CD DG44 medium is a commercial product. The precise formulation is proprietary.
https://link.springer.com/content/pdf/10.1007%2Fs12033-017-0037-4.pdf |
|
Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |
Contains phenol red > No | No |
Antibiotics free > Yes | Yes |