Cell line/type | DPCs (Dental pulp cells) |
---|---|
Species | Human |
Animal free | Yes |
Product | DMEM Ishkitiev et al. 2012 |
Ishkitiev, N., Yaegaki, K., Imai, T., Tanaka, T., Nakahara, T., Ishikawa, H., ... & Haapasalo, M. (2012). High-purity hepatic lineage differentiated from dental pulp stem cells in serum-free medium. Journal of endodontics, 38(4), 475-480. In this study, the objective was to investigate the capacity for and purity of hepatocyte-like differentiation of CD117-positive dental pulp stem cells without serum. Mesenchymal cells from human deciduous and extracted third molar pulp were isolated and expanded in vitro. For hepatic differentiation, the media were supplemented with hepatic growth factor, insulin-transferrin-selenium-x, dexamethasone, and oncostatin M. Without serum both cell types differentiated into high-purity hepatocyte-like cells. These cells offer a source for hepatocyte lineage differentiation for transplantation in the future. Cells were cultured in serum-free, DMEM based, medium supplemented with different components as specified in the Materials and Methods, under the heading; Hepatogenic Differentiation. https://ac.els-cdn.com/S1465324912706217/1-s2.0-S1465324912706217-main.pdf?_tid=de431f26-ce24-4d37-810b-0b68a4889d08&acdnat=1533551286_2e332abf8594cb1c32fb47ffe2e0093e |
|
Source | Literature - own formulation |
Chemically defined > Unspecified | Unspecified |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |