Cell line/type | DPCs (Dental pulp cells) |
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Species | Human |
Animal free | Yes |
Product | PRIME-XV® MSC Expansion XSFM |
Mochizuki, M., & Nakahara, T. (2018). Establishment of xenogeneic serum-free culture methods for handling human dental pulp stem cells using clinically oriented in-vitro and in-vivo conditions. Stem cell research & therapy, 9(1), 25. In this study, dental pulp stem cells were derived from wisdom teeth donated by eight healthy volunteers and cultured in PRIME-XV® MSC Expansion XSFM, a xenogeneic serum-free culture medium (XFM), or xenogeneic serum-containing culture medium (SCM). In primary culture, XFM cells showed lower adhesion and slightly different morphology, although the single-cell size was similar to that of SCM cells. XFM cells exhibited typical mesenchymal stem cell (MSC) characteristics in vitro and in vivo, including marker gene/protein expression, trilineage differentiation potential, and hard, osteo-dentin tissue formation. Additionally, XFM cells maintained a normal karyotype in vitro and nontumorigenic potential in vivo; however, XFM cells were more susceptible to H2O2 and ultraviolet cytotoxic stimuli. XFM cells formed a multilayered structure showing excessive cell death/division in contrast to the monolayered structure of SCM cells when reaching overconfluence. Proliferation was disrupted in overconfluent XFM cells, and these cells could not be subcultured. Dimethyl sulfoxide-free cryopreserved XFM cells yielded similar results in all of the experiments. In conclusion, successful isolation and expansion of an MSC population from donor-derived tissue (dental pulp) under xenogeneic serum-free culture conditions was reported, as well as the application of cryopreservation, using a research strategy based on clinically oriented in-vitro and in-vivo experiments. PRIME-XV® MSC Expansion XSFM is a commercial product. The precise formulation is proprietary.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5797401/pdf/13287_2017_Article_761.pdf |
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Source | Literature - commercial |
Chemically defined > Unspecified | Unspecified |