Cell line/type | DPCs (Dental pulp cells) |
---|---|
Species | Human |
Animal free | Yes |
Product | DMEM/F12-bFGF Morito et al. 2009 |
Morito, A., Kida, Y., Suzuki, K., Inoue, K., Kuroda, N., Gomi, K., ... & Sato, T. (2009). Effects of basic fibroblast growth factor on the development of the stem cell properties of human dental pulp cells. Archives of histology and cytology, 72(1), 51-64. In this study, human dental pulp cells (hDPCs) were cultured in a serum-free medium containing bFGF. The hDPCs strongly expressed STRO-1 immunoreactive products and sustained self-renewal, and thus were almost identical in differentiation potential and proliferation activity to hDPCs cultured with the medium containing serum and bFGF. The results suggest that the hDPCs cultured in the presence of bFGF irrespective of the presence or absence of the bovine serum are rich in mesenchymal stem cells or progenitor cells and useful for cell-based therapies to treat dental diseases. Serum-free DMEM/F12 was supplemented with different components as specified in the Materials and Methods, under the heading; Samples and cell cultures. https://www.jstage.jst.go.jp/article/aohc/72/1/72_1_51/_pdf |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |