Cell line/type | DPCs (Dental pulp cells) |
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Species | Human |
Animal free | Yes |
Product | STK2 Fujii et al. 2018 |
Fujii, S., Fujimoto, K., Goto, N., Abiko, Y., Imaoka, A., Shao, J., ... & Nishimura, F. (2018). Characterization of human dental pulp cells grown in chemically defined serum‑free medium. Biomedical reports, 8(4), 350-358. This study examined whether dental pulp cells (DPCs) could be expanded under serum-free conditions. DPCs obtained from four donors were identified to proliferate actively in the serum-free medium, STK2, when compared with those cells in control medium (Dulbecco's modified Eagle's medium containing 10% serum). The high proliferative potential with STK2 was maintained through multiple successive culture passages. DNA microarray analyses demonstrated that the gene expression profile of DPCs grown in STK2 was similar to that of cells grown in the control medium; however, a number of genes related to cell proliferation, including placental growth factor and inhibin-betaE, were upregulated in the STK2 cultures. Following induction of osteogenesis, DPCs grown in STK2 induced alkaline phosphatase activity and calcification at higher levels compared with the control medium cultures, indicating maintenance of differentiation potential in STK2. The precise formulation of STK2 medium is proprietary. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5844140/pdf/br-08-04-0350.pdf |
|
Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |