Cell line/type | ImEry (Erythroblast cell line) |
---|---|
Species | Human |
Animal free | Yes |
Product | OpIMDM Lee et al 2018 |
Lee, E., Lim, Z. R., Chen, H. Y., Yang, B. X., Lam, A. T. L., Chen, A. K. L., ... & Oh, S. K. W. (2018). Defined SerumāFree Medium for Bioreactor Culture of an Immortalized Human Erythroblast Cell Line. Biotechnology journal, 13(4), 1700567. In this study, the authors use an erythroblast cell line (ImEry) derived from immortalizing CD71+CD235a+ erythroblast from adult peripheral blood for optimization of expansion culture conditions. Design of experiments is used in media formulation to explore relationships and interactive effects between factors which affect cell expansion. The in-house optimized medium formulation produced significantly higher cell densities (3.62 +/- 0.055) x 10(6) cells mL(-1) , n = 3) compared to commercial formulations (2.07 +/- 0.055) x 10(6) cells mL(-1) , n = 3; at 209 h culture). The formulation of OpIMDM can be found in the Results, under the heading Media Formulation and Optimization Using DOE Software. https://onlinelibrary.wiley.com/doi/pdf/10.1002/biot.201700567 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Antibiotics free > No | No |
Contains phenol red > Yes | Yes |