Cell line/type | T cells |
---|---|
Species | Human |
Animal free | Yes |
Product | CTS OpTmizer T-cell Expansion SFM |
Smith, C., Økern, G., Rehan, S., Beagley, L., Lee, S. K., Aarvak, T., ... & Khanna, R. (2015). Ex vivo expansion of human T cells for adoptive immunotherapy using the novel Xeno‐free CTS Immune Cell Serum Replacement. Clinical & translational immunology, 4(1), e31. In this study a novel xeno-free serum replacement (SR) with defined components that can be reproducibly used for the production of clinical grade T-cell therapies in combination with several different cell culture media is described. Dynabeads CD3/CD28 Cell Therapy System (CTS)-activated or antigen-specific T cells expanded using the xeno-free SR, CTS Immune Cell SR, showed comparable growth kinetics observed with cell culture media supplemented with HS or FBS. Furthermore, T cells expanded using xeno-free SR medium were highly amenable to lentivirus-mediated gene transduction for potential application for gene-modified T cells. CTS OpTmizer T-cell Expansion SFM is a commercial product. The precise formulation is proprietary. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4318490/pdf/cti201431a.pdf |
|
Source | Literature - commercial |
Chemically defined > Yes | Yes |
Antibiotics free > Yes | Yes |
Contains phenol red > Yes | Yes |