Cell line/type | T cells |
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Species | Human |
Animal free | Yes |
Product | X-VIVO 15 and 1B2H |
Medvec, A. R., Ecker, C., Winters, E. A., Glover, J., Varela-Rohena, A., & Riley, J. L. (2018). Improved expansion and in vivo function of patient T cells by a serum-free medium. Molecular Therapy-Methods & Clinical Development, 8, 65-74. In this study, a recently developed chemically defined cell culture medium that robustly expands all T cell subsets in the absence of human serum was used. Using a humanized mouse model, it was observed that T cells expanded in the absence of human serum provided durable control of tumors, whereas T cells expanded in medium supplemented with human serum only mediated transient control of tumor growth. The new medium effectively expanded more differentiated T cells from multiple myeloma patients in the absence of serum. These patient-derived T cells were also able to provide durable control of B cell tumors in vivo, and this long-term control of cancer was lost when T cells were expanded in the presence of serum. In this study, both X-VIVO 15 and 1B2H supported T cell expansion nearly equivalently in the presence and absence of serum, although it is important to note that T cells expanded in the absence of serum rested more quickly than T cells expanded in the presence of serum. Thus, both X-VIVO 15 and 1B2H fulfill the requirement of being a bona fide serum-free medium: similar expansion in the presence and absence of human serum. The precise formulations of both of X-VIVO 15 and 1B2H media are proprietary. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5907749/pdf/main.pdf |
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Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |