Cell line/type | Kidney organoids |
---|---|
Species | Human |
Animal free | No |
Product | KD/F12 + KSR Nishikawa et al. 2018 |
Nishikawa, M., Kimura, H., Yanagawa, N., Hamon, M., Hauser, P., Zhao, L., ... & Yanagawa, N. (2018). An optimal serum-free defined condition for in vitro culture of kidney organoids. Biochemical and biophysical research communications, 501(4), 996-1002. In this study, the authors tested two serum-free culture conditions and compared their suitability for the maintenance and growth of kidney organoids in culture. One of the serum-free culture conditions was the combination of keratinocytes serum-free medium (KSFM) with knockout serum replacement (KSR) (KSFM + KSR), and the other was the combination of knockout DMEM/F12 (KD/F12) and KSR (KD/F12 + KSR). With cell aggregates derived from E12.5 mouse embryonic kidneys, it was found that KD/F12 + KSR was superior to KSFM + KSR in promoting the growth of the aggregate with expansion of Six2(+) nephron progenitor cells (NPC) and elaborated ureteric branching morphogenesis. With KD/F12 + KSR, it was found that lower concentrations of KSR at 5-10% were superior to a higher concentration (20%) in promoting the growth of aggregates without affecting the expression levels of NPC marker genes. The authors also found that NPC in aggregates retained their differentiation potential to develop nephron tubules through mesenchyme-to-epithelial transition (MET), after being maintained in culture under these conditions for up to 7 days. In conclusion, a defined serum-free culture condition suitable for the maintenance and growth of kidney organoids that retain the differentiation potential to develop nephron structures was identified. The media formulation of KD/F12 + KSR is listed in Table 1.
https://ac.els-cdn.com/S0006291X18311616/1-s2.0-S0006291X18311616-main.pdf?_tid=c24ef524-c91d-49c9-9652-4282e53c7efa&acdnat=1530019761_96c25054d6f2dacf74ee1be97e06a64e |
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Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |