Cell line/type | SHED (Stem cells from human exfoliated deciduous teeth) |
---|---|
Species | Human |
Animal free | No |
Product | K-M |
Tarle, S. A., Shi, S., & Kaigler, D. (2011). Development of a serum‐free system to expand dental‐derived stem cells: PDLSCs and SHEDs. Journal of cellular physiology, 226(1), 66-73. In this study, the development of a chemically defined, serum-free media (K-M) was investigated for the expansion of human periodontal ligament stem cells (PDLSCs) and human stem cells from exfoliated deciduous teeth (SHEDs). Proliferation assays were performed comparing cells in serum-containing media (FBS-M) with cells cultured in four different serum-free medium and these demonstrated that in these media, the cell proliferation of both cell types was significantly less than the proliferation of cells in FBS-M. Additional proliferation assays were performed using pre-coated fibronectin (FN) tissue culture plates and of the four serum-free medium, only K-M enabled PDLSCs and SHEDs to proliferate at higher rates than cells cultured in FBS-M. Next, alkaline phosphatase activity showed that PDLSCs and SHEDs exhibited similar osteogenic potential whether cultured in K-M or FBS-M, and, additionally, cells retained their multipotency in K-M as seen by expression of chondrogenic and adipogenic genes, and positive Von Kossa, Alcian blue, and Oil Red O staining. Finally, differential expression of 84 stem cell associated genes revealed that for most genes, PDLSCs and SHEDs did not differ in their expression regardless of whether cultured in K-M or FBS-M. Taken together, the data suggest that K-M can support the expansion of PDLSCs and SHEDs and maintenance of their multipotency. The formulation of K-M medium is listed in the Materials and Methods, under the heading; Cell culture. https://deepblue.lib.umich.edu/bitstream/handle/2027.42/78237/22304_ftp.pdf?sequence=1 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > No | No |
Antibiotics free > No | No |