|Cell line/type||Renal stem/progenitor cells|
Hu, K., Denk, L., de Vries, U., & Minuth, W. W. (2007). Chemically defined medium environment for the development of renal stem cells into tubules. Biotechnology Journal: Healthcare Nutrition Technology, 2(8), 992-995. doi: 10.1002/biot.200700009
In this study, embryonicc renal tissue containing stem/progenitor cells was mounted within a perfusion culture container at the interface of an artificial interstitium made of polyester. Using this innovative approach the authors show that renal tubules develop in chemically defined Iscove's modified Dulbecco's medium without serum addition and without coating by extracellular matrix proteins. Although these cultures could be maintained for weeks in chemically defined medium, the quality and quantity of generated tubules look promising for future laboratory work.
The new culture technology and methods, using chemically defined IMDM-HEPES media, is described in this article.
|Source||Literature - own formulation|
|Chemically defined > Yes||Yes|
|Contains phenol red > Yes||Yes|
|Antibiotics free > Yes||Yes|