Cell line/type | Oocyte |
---|---|
Species | Bovine (cow) |
Animal free | Yes |
Product | McCoy’s 5a McLaughlin et al. 2010 |
McLaughlin, M., & Telfer, E. E. (2010). Oocyte development in bovine primordial follicles is promoted by activin and FSH within a two-step serum-free culture system. Reproduction, 139(6), 971-978. The aims of this study were 1) to determine whether bovine follicles initiated to grow in vitro could be isolated from the cortical environment, and could undergo further development and 2) to evaluate the effect of activin and FSH on the development of secondary follicles derived from primordial follicles. Fragments of bovine ovarian cortex were cultured in serum-free medium for 6 days; thereafter, secondary follicles were isolated for further culture. In summary, this study demonstrates the promotion of primordial bovine follicle development within a two-step serum-free culture system with oocyte diameters O100 mm achieved over 15 days in vitro. McCoy’s 5a medium was supplemented with different components as specified in the Materials and Methods, under the heading; Tissue preparation and fragment culture. The formulation of McCoys 5A medium can be found online: https://www.atcc.org/~/media/Attachments/E/D/8/C/6734.ashx http://www.reproduction-online.org/content/139/6/971.full.pdf |
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Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |