Cell line/type | Oocyte |
---|---|
Species | Bovine (cow) |
Animal free | Yes |
Product | KSOM-PVA |
Bhuiyan, M. U., Kang, S. K., & Lee, B. C. (2007). Supplementation of fructose in chemically defined protein-free medium enhances the in vitro development of bovine transgenic cloned embryos. Zygote, 15(3), 189-198. This study evaluated the possible embryotrophic role of fructose supplementation in chemically defined protein-free KSOM on in vitro development of bovine transgenic cloned embryos. The authors concluded that supplementation of combined fructose (1.5 mM) and glucose (0.2 mM) in chemically defined protein-free KSOM, KSOM supplemented with 0.01% PVA instead of BSA, enhances the in vitro development of bovine transgenic cloned embryos.
Bhuiyan, M. M. U., Kang, S. K., & Lee, B. C. (2007). Effects of fructose supplementation in chemically defined protein-free medium on development of bovine in vitro fertilized embryos. Animal reproduction science, 102(1-2), 137-144. This study by the same authors evaluated the possible embryotrophic role of fructose supplementation in potassium simplex optimization medium (KSOM) on preimplantation development of bovine in vitro matured and fertilized (IVF) embryos under chemically defined conditions. This study indicates that fructose up to 5.6 mM concentration can be used as an alternative for energy substrate in culture media without any detrimental effect on pre-implantation development in bovine IVF embryos.
The formulation of KSOM can be found online.--> http://cshprotocols.cshlp.org/content/2006/1/pdb.rec10404.full?text_only=true https://www.cambridge.org/core/services/aop-cambridge-core/content/view/AFBBCA02259064E57E2028B7DD7ED7A7/S0967199407004236a.pdf/div-class-title-supplementation-of-fructose-in-chemically-defined-protein-free-medium-enhances-the-span-class-italic-in-vitro-s |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Antibiotics free > No | No |