Cell line/type | Oocyte |
---|---|
Species | Porcine (Pig) |
Animal free | Yes |
Product | POM-PVA |
Appeltant, R., Somfai, T., Nakai, M., Bodó, S., Maes, D., Kikuchi, K., & Van Soom, A. (2015). Interactions between oocytes and cumulus cells during in vitro maturation of porcine cumulus-oocyte complexes in a chemically defined medium: effect of denuded oocytes on cumulus expansion and oocyte maturation. Theriogenology, 83(4), 567-576. The aim of this study was to clarify interactions between oocytes and cumulus cells on the level of cumulus expansion and oocyte maturation during IVM of cumulus-oocyte complexes (COCs) in a chemically defined medium using a system that allows individual tracking of oocytes. Especially, the influence of oocyte-secreted factors was investigated by the aid of addition of denuded oocytes as a possible approach to improve the IVM system. The basic maturation medium was porcine oocyte medium with addition of gonadotropins only during the first 20 hours of IVM. Cumulus expansion in a porcine oocyte medium of intact COCs increased in a typical manner until 20 hours and decreased in size subsequently until 48 hours of IVM (P < 0.05). The base medium POM is was supplemented with different components as specified in the Materials and Methods. The formulation of POM is listed in Table 1 of a previous article, Yoshioka et al. 2008. |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > No | No |
Antibiotics free > No | No |