|Product||Modified NCSU-23 medium|
Hong, J., & Lee, E. (2007). Intrafollicular amino acid concentration and the effect of amino acids in a defined maturation medium on porcine oocyte maturation, fertilization, and preimplantation development. Theriogenology, 68(5), 728-735.
The objective of this study was to determine the intrafollicular concentrations of free amino acids in pigs and to examine the effect of amino acids in a chemically defined maturation medium on oocyte maturation, in vitro fertilization (IVF), and embryo development in vitro. Pooled follicular fluid aspirated separately from small (<3mm in diameter), medium (3-8mm), and large follicles (>8mm) in three pairs of ovaries was analyzed for amino acid concentration. In addition, oocyte maturation, fertilization, and embryo development were examined after in vitro maturation (IVM) of oocytes in a defined maturation medium supplemented individually with glutamate (GLU), glutamine (GLN), glycine (GLY), aspartate (ASP), asparagine (ASN), arginine (ARG), alanine (ALA), leucine (LEU), lysine (LYS), proline (PRO), and valine (VAL). Irrespective of follicle size, GLY, GLU, ALA, GLN, and PRO were the most abundant amino acids in pig follicular fluid (pFF) and amino acids in a defined medium improved porcine monospermic fertilization, male pronuclear formation, and preimplantation development.
The adaptations to the NCSU-23 medium are described in the Materials and Methods, under the headings, Embryo culture and Experimental design and statistical analysis. The formula of the NCSU-23 medium is listed in a previous article. --> Peters RM, Wells KD. Culture of pig embryos. J. Reprod. Fertil. 1993;48(Suppl):61–73.https://ac.els-cdn.com/S0093691X07003287/1-s2.0-S0093691X07003287-main.pdf?_tid=84e04fae-0ae3-4105-aadc-65e4ad66845a&acdnat=1530709966_2078bbd3c4f21c1168619d201d3d7003
|Source||Literature - own formulation|
|Chemically defined > Yes||Yes|
|Contains phenol red > No||No|
|Antibiotics free > No||No|