|Product||Modified medium 199 Ishizaki et al. 2009|
Ishizaki, C., Watanabe, H., Bhuiyan, M. M. U., & Fukui, Y. (2009). Developmental competence of porcine oocytes selected by brilliant cresyl blue and matured individually in a chemically defined culture medium. Theriogenology, 72(1), 72-80.
The objective of this study was to investigate the effects of oocyte selection using brilliant cresyl blue (BCB) and culture density during individual in vitro maturation (IVM) on porcine oocyte maturity and subsequent embryo development using a chemically defined medium. A chemically defined media was used in all culture steps. Modified medium 199 was used for oocyte maturation and PZM-4 for embryo culture.
The formula is listed in the Materials and Methods, under the heading Oocyte maturation and In vitro embryo culture. The formula of medium 199 can be found online. The formula of PZM-4 is listed in a former article.
|Source||Literature - own formulation|
|Chemically defined > Yes||Yes|
|Contains phenol red > No||No|
|Antibiotics free > No||No|