Cell line/type | Oocyte |
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Species | Porcine (Pig) |
Animal free | Unspecified |
Product | mPOM |
Koike, T., Matsuura, K., Naruse, K., & Funahashi, H. (2010). In-vitro culture with a tilting device in chemically defined media during meiotic maturation and early development improves the quality of blastocysts derived from in-vitro matured and fertilized porcine oocytes. Journal of Reproduction and Development, 56(5), 552-557. This study was undertaken to examine the effects of kinetic culture with a tilting device in chemically defined media during in vitro maturation (IVM) of porcine oocytes and in vitro culture (IVC) following in vitro fertilization (IVF) on the early developmental competence and quality of blastocysts. The authors concluded that current observations indicate that tilting culture during IVM and in vitro culture following IVF in chemically defined media improves the quality of blastocyst without any effects on the developmental competence, although embryos may be more sensitive to the beneficial shear stress, as compared with COC. The precise formulation is proprietary. mPOM is POM supplemented with 50 μM beta-mercaptoethanol. POM is developed by the Research Institute for the Functional Peptides, Yamagata, Japan. http://ousar.lib.okayama-u.ac.jp/files/public/4/48975/20160528093535182303/JReproductionDevelopment_56_5_552-557.pdf |
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Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |