Cell line/type | Oocyte |
---|---|
Species | Canine (Dog) |
Animal free | No |
Product | mSOF Salavati et al. 2012 |
Salavati, M., Ghafari, F., Zhang, T., & Fouladi-Nashta, A. A. (2012). Effects of oxygen concentration on in vitro maturation of canine oocytes in a chemically defined serum-free media. Reproduction, REP-12.
The base maturation media was modified synthetic oviductal fluid (mSOF) supplemented with different components as specified in the Materials and Methods, under the heading; In vitro maturation. The formulation of mSOF is listed in Table 1 of a previous article, Holm et al. 1999. Holm P, Booth PJ, Schmidt MH, Greve T & Callesen H 1999 High bovine blastocyst development in a static in vitro production system using SOFaa medium supplemented with sodium citrate and myo-inositol with or without serum-proteins. Theriogenology 52 683–700. (doi:10.1016/ S0093-691X(99)00162-4 http://www.reproduction-online.org/content/144/5/547.full.pdf |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |