Cell line/type | L929 cells |
---|---|
Species | Mouse |
Animal free | Yes |
Product | DMEM Karakecili et al. 2007 |
Karakecili, A. G., Demirtas, T. T., Satriano, C., Gümüsderelioglu, M., & Marletta, G. (2007). Evaluation of L929 fibroblast attachment and proliferation on Arg-Gly-Asp-Ser (RGDS)-immobilized chitosan in serum-containing/serum-free cultures. Journal of bioscience and bioengineering, 104(1), 69-77. In this study, chitosan membranes prepared by the solvent casting method were modified with the Arg-Gly-Asp-Ser (RGDS) sequence of fibronectin using the photochemical immobilization technique. In vitro cell culture studies were performed with L929 mouse fibroblasts to investigate the effect of biomodification on fibroblast cell behaviour in serum-free and 10% serum-containing media. The results obtained from cell culture studies pointed out the specific interactions between biosignal RGDS molecules and fibroblast cells. A triggered cell attachment and proliferation were observed on RGDS-modified chitosan membranes that were more distinguishable in serum-free medium. Cell attachment experiments were (also) conducted in DMEM without serum, as specified in the Materials and Methods, under the heading; Cell culture studies. https://ac.els-cdn.com/S1389172307701214/1-s2.0-S1389172307701214-main.pdf?_tid=3dfd475c-a278-4046-882f-76d4850ad127&acdnat=1534430145_a7bf4f30e69fc5434442fc656aabb483 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |