Cell line/type | HFF (Human Foreskin Fibroblasts) |
---|---|
Species | Human |
Animal free | Yes |
Product | Stripped Green’s Plus |
Mujaj, S., Manton, K., Upton, Z., & Richards, S. (2010). Serum-free primary human fibroblast and keratinocyte coculture. Tissue Engineering Part A, 16(4), 1407-1420. In this study, a medium was developed that used recombinant proteins to support the serum-free isolation and expansion of human dermal fibroblasts and keratinocytes. The human dermal fibroblasts were able to be isolated serum free by adding recombinant human albumin to a collagenase solution. These fibroblasts were then expanded using a serum-free medium containing recombinant proteins: epidermal growth factor, basic fibroblast growth factor, chimeric vitronectin:insulin-like growth factor-I protein, and recombinant human albumin. These fibroblasts maintained a typical morphology and expressed fibroblast markers during their serum-free isolation, expansion, and freezing. Moreover, these fibroblasts were able to support the serum-free isolation and expansion of primary keratinocytes using these recombinant proteins. Real-time polymerase chain reaction and immunofluorescence analysis confirmed that there were no differences in expression levels of p63 or keratins 1, 6, and 10 when keratinocytes were grown in either serum-supplemented or serum-free medium. The keratinocytes maintained their ability to reform an epidermal layer. In summary, the techniques described provide a valuable alternative for culturing fibroblasts and keratinocytes using recombinant proteins. The formulation of Stripped Green’s Plus is listed in the Materials and Methods, under the heading; Culture of HFF cell lines. https://www.liebertpub.com/doi/pdfplus/10.1089/ten.tea.2009.0251 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |