Cell line/type | HFF (Human Foreskin Fibroblasts) |
---|---|
Species | Human |
Animal free | Yes |
Product | Modified DMEM/F12 3:1 Throm et al. 2010 |
Throm, A. M., Liu, W. C., Lock, C. H., & Billiar, K. L. (2010). Development of a cell‐derived matrix: Effects of epidermal growth factor in chemically defined culture. Journal of Biomedical Materials Research Part A: An Official Journal of The Society for Biomaterials, The Japanese Society for Biomaterials, and The Australian Society for Biomaterials and the Korean Society for Biomaterials, 92(2), 533-541. In a previous study, the authors demonstrated that CDM developed in chemically defined media supplemented with 5 ng/mL EGF is stronger, is produced more rapidly, and is biochemically more similar to native skin than when grown in standard serum-containing medium. The results of this study confirmed the superiority of chemically defined media over serum-supplemented media. The formula of the chemically defined media is listed in the Methods, under the heading Cell culture. https://onlinelibrary.wiley.com/doi/pdf/10.1002/jbm.a.32369 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Antibiotics free > No | No |
Contains phenol red > No | No |