|Cell line/type||HFF (Human Foreskin Fibroblasts)|
|Product||Modified DMEM/F12 3:1 Throm et al. 2010|
Throm, A. M., Liu, W. C., Lock, C. H., & Billiar, K. L. (2010). Development of a cell‐derived matrix: Effects of epidermal growth factor in chemically defined culture. Journal of Biomedical Materials Research Part A: An Official Journal of The Society for Biomaterials, The Japanese Society for Biomaterials, and The Australian Society for Biomaterials and the Korean Society for Biomaterials, 92(2), 533-541.
In a previous study, the authors demonstrated that CDM developed in chemically defined media supplemented with 5 ng/mL EGF is stronger, is produced more rapidly, and is biochemically more similar to native skin than when grown in standard serum-containing medium. The results of this study confirmed the superiority of chemically defined media over serum-supplemented media.
The formula of the chemically defined media is listed in the Methods, under the heading Cell culture.
|Source||Literature - own formulation|
|Chemically defined > Yes||Yes|
|Antibiotics free > No||No|
|Contains phenol red > No||No|