Cell line/type | Blastocysts |
---|---|
Species | Mouse |
Animal free | No |
Product | Mouse embryonic fibroblast-conditioned medium |
Liu, X., Wei, Q., Zhang, J., Yang, W., Zhao, X., & Ma, B. (2015). Derivation of embryonic stem cells from Kunming mice IVF blastocyst in feeder-and serum-free condition. In Vitro Cellular & Developmental Biology-Animal, 51(6), 541-545. In this study, two novel ESC lines were established from IVF blastocyst of Kunming mice in a feeder- and serum-free condition containing 2.5 muM 6-Bromoindirubin-3'-oxime (BIO). Two Kunming mice ESC lines, designated as KMES1 and KMES2, were obtained. The formulation of the mouse embryonic fibroblast-conditioned medium (CM) is specified in the Materials and Methods, under the heading; Embryonic stem cell derivation and culture. https://link.springer.com/content/pdf/10.1007%2Fs11626-014-9863-x.pdf |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |