Cell line/type | Blastocysts |
---|---|
Species | Porcine (Pig) |
Animal free | Yes |
Product | PBM/PZM-5 |
Mito, T., Yoshioka, K., Noguchi, M., Yamashita, S., Misumi, K., Hoshi, T., & Hoshi, H. (2015). Birth of piglets from in vitro–produced porcine blastocysts vitrified and warmed in a chemically defined medium. Theriogenology, 84(8), 1314-1320. In this study, the effect of different embryo developmental stages, culture periods, and media on the cryotolerance of in vitro-produced porcine blastocysts was examined. All media used for in vitro production, vitrification, and warming were chemically defined. When in vitro-produced embryos at the blastocyst and expanded blastocyst stages were vitrified using the Cryotop method on Day 5 or 6 (Day 0 = the day of IVF), the survival rate and hatching rate of expanded blastocysts after warming were higher than those of blastocysts. The viability after vitrification and warming of Day-6 embryos cultured in porcine blastocyst medium from Day 5 were higher than that of embryos cultured in porcine zygote medium 5. On the other hand, there were no significant differences on the cryotolerance between Day-5 embryos cultured in porcine zygote medium 5 and those replaced with porcine blastocyst medium on Day 4. There were no significant differences in viability between the embryonic ages of 5 and 6 days after vitrification and warming. When expanded blastocysts vitrified on Day 5 or 6 were surgically transferred to recipient gilts, all three recipients became pregnant in the Day-5 group, whereas only one out of three recipients became pregnant in the Day-6 group. These results indicate that the cryotolerance of porcine in vitro-produced blastocysts after vitrification appears to depend on the embryonic stage, culture period, and medium. In Table 1 Composition of culture media for in vitro production of porcine embryos, the precise formulations of the examined chemically defined media, PBM and PZM-5, are listed. https://ac.els-cdn.com/S0093691X15003532/1-s2.0-S0093691X15003532-main.pdf?_tid=e450b026-15cd-409a-9180-e03c60119dd3&acdnat=1530020262_8b2e57d399afabbac0fa857c045d9863 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Antibiotics free > No | No |
Contains phenol red > No | No |