Cell line/type | Follicle |
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Species | Sheep |
Animal free | Yes |
Product | TCM199B Arunakumari et al. 2010 |
Arunakumari, G., Shanmugasundaram, N., & Rao, V. H. (2010). Development of morulae from the oocytes of cultured sheep preantral follicles. Theriogenology, 74(5), 884-894. In this study, sheep preantral follicles (PFs) measuring 250-400 microm in diameter were cultured for six days in serum-free media supplemented differently with growth factors and hormones. Based on the proportion of the PFs exhibiting growth, mean increase in diameter, proportions of PFs developing antrum, ovulations in vitro and oocytes maturing to M-II stage, 1% ITS, 10 ng/mL each of IGF-I, and Insulin and 1 mIU/mL of GH were found to support the best development of sheep PFs. However, the oocytes from PFs cultured in any concentration of TGF-beta failed to mature to M-II stage. Similarly, among the combinations studied, IGF-I+GH was found to be the best. In combination with T4 and FSH, IGF-I+GH supported the best development of the PFs. Culture of PFs in micro drops or agar gel supported similarly high development. In vitro fertilization of the oocytes from the cultured sheep PFs resulted in the embryos developing to the morula stage for the first time. The base medium TCM199H, HEPES buffered tissue culture medium 199, was supplemented with different components as specified in the Materials and Methods, under the heading; 2.2. Culture of preantral follicles and assessment of the development, and in table 1-3. The formulation of medium 199 can be found online: https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Formulation/m0650for.pdf. https://www.sciencedirect.com/science/article/pii/S0093691X10002207 |
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Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |