|Product||aMEM Gook et al. 2014|
Gook, D. A., Edgar, D. H., Lewis, K., Sheedy, J. R., & Gardner, D. K. (2013). Impact of oxygen concentration on adult murine pre-antral follicle development in vitro and the corresponding metabolic profile. Molecular human reproduction, 20(1), 31-41.
The aim of this study was to establish a defined culture environment for adult mouse primary and secondary follicles in which the impact of oxygen concentration could be assessed at both the morphological and metabolic levels. A secondary aim was to determine whether frequent medium replenishment is necessary with the use of a more stable form of glutamine. In this study, mechanically isolated primary and secondary follicles (80-140 microm diameter) from adult mouse ovaries were cultured in serum-free conditions for 8 days in either 5 or 20% oxygen to determine growth (follicular diameter), morphology and viability. In conclusion, the poor in vitro follicular development previously observed in minimal culture conditions may reflect the use of 20% oxygen. Frequent medium replenishment is not necessary and does not overcome the detrimental effect of high oxygen on follicle viability. Further optimization of culture conditions would benefit from metabolic analyses and the use of 5% oxygen should be tested further for impact on functional aspects of follicle culture such as steroid production which is currently unknown.
The culture medium used in this study is serum-free aMEM supplemented human serum albumin and other components as specified in the Materials and Methods, under the heading; Culture medium.
|Source||Literature - own formulation|
|Chemically defined > Yes||Yes|
|Contains phenol red > Yes||Yes|
|Antibiotics free > No||No|