Park, Y. H., Gong, S. P., Kim, H. Y., Kim, G. A., Choi, J. H., Ahn, J. Y., & Lim, J. M. (2013). Development of a serum‐free defined system employing growth factors for preantral follicle culture. Molecular reproduction and development, 80(9), 725-733.
This study was conducted to evaluate if mouse preantral follicles can yield developmentally competent oocytes following culture in serum-free, defined medium. Donor follicles were obtained from 14-day-old B6CBAF1 mice, and cultured in alpha-MEM-Glutamax medium. The authors conclude that serum-free media can be used to optimize ovarian follicle cultures, and the addition of SCF is beneficial for deriving developmentally competent oocytes through follicle culture.
The formulation of alpha-MEM-Glutamax is available online --> (https://www.thermofisher.com/nl/en/home/technical-resources/media-formulation.109.html). KSR is a commercial media supplement.https://onlinelibrary.wiley.com/doi/pdf/10.1002/mrd.22204
|Source||Literature - modified commercial product|
|Chemically defined > Yes||Yes|
|Antibiotics free > Yes||Yes|
|Contains phenol red > Yes||Yes|