Cell line/type | Follicle |
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Species | Feline (cat) |
Animal free | Yes |
Product | MEM-PVA Fujihara et al. 2012 |
Fujihara, M., Comizzoli, P., Wildt, D. E., & Songsasen, N. (2012). Cat and dog primordial follicles enclosed in ovarian cortex sustain viability after in vitro culture on agarose gel in a proteināfree medium. Reproduction in Domestic Animals, 47, 102-108. The objective of this study was to examine the influences of differing media, protein supplementation and the microenvironment on cat vs dog primordial follicle viability in vitro. Ovarian cortical slices were cultured for 3, 9 or 15 days in alpha-minimum essential medium (alpha-MEM) or MEM supplemented with 10% fetal bovine serum (FBS), 10% knock-out serum replacement (KSR) or 0.1% polyvinyl alcohol (protein free). No cat follicle sustained viability beyond 9 days of in vitro culture in alpha-MEM compared to 37.5% of those incubated for 15 days in MEM in protein-free condition (p < 0.05). In contrast, alpha-MEM was superior (p < 0.05) to MEM in maintaining dog follicle viability (32.7% vs 8.1%) in protein-free condition at 15 days. Serum was detrimental (p < 0.05) to follicle survival in both species. Knock-out serum replacement supplementation and a protein-free condition supported cat follicle viability, whereas the latter was superior (p < 0.05) to the former for sustaining dog follicle survival. Likewise, dog follicle viability was enhanced (p < 0.05) by the agarose gel compared to the cell culture insert and control groups after 3 and 9 days of culture. For the cat, the agarose gel better (p < 0.05) supported follicle viability compared to the control, but was equivalent to the cell culture insert. Therefore, sustaining primordial follicle survival from intracortical ovarian slices requires a different in vitro microenvironment for the cat vs the dog. A key factor to enhancing survival of these early stage follicles in culture appears to be the use of agarose gel, which enhances follicle viability, perhaps by promoting gas exchange. Interestingly, both cat and dog primordial follicles tolerated and even preferred a protein-free culture medium. The formulation of alpha-MEM-PVA (dog) and MEM-PVA (cat) is listed in the Experimental design Study 1: Influence of in vitro culture medium and protein supplementation on follicle viability. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3965327/pdf/nihms553151.pdf |
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Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |