Cell line/type | CHO (Chinese Hamster Ovary) CHO-K1 |
---|---|
Species | Hamster |
Animal free | Yes |
Product | HyQ PF-CHO MPS Leong et al. 2017 |
Leong, D. S. Z., Tan, J. G. L., Chin, C. L., Mak, S. Y., Ho, Y. S., & Ng, S. K. (2017). Evaluation and use of disaccharides as energy source in protein-free mammalian cell cultures. Scientific Reports, 7, 45216. In this study, it was found that mammalian cells can utilize maltose for growth in the absence of glucose and successfully adapted CHO-K1, CHO-DG44 and HEK293 cells to grow in glucose-free, maltose-containing serum-free protein-free media. We then cultivated a non-adapted CHO-K1 producer cell line in media containing both glucose and maltose to show that the cells can utilize maltose in a biphasic manner, that maltose enters the cells, and that maltose utilization only took place in the presence of the cells. It was demonstrated that mammalian cells can proliferate in serum-free protein-free culture medium utilizing maltose, but not sucrose, lactose or trehalose, as sugar source.This is the first report of a protein-free mammalian cell culture using a disaccharide as energy source. CHO-K1, was cultivated using a seeding cell density of 0.3 × 106 cells/ml, with 3.6 g/l of maltose, sucrose, lactose, trehalose or glucose as energy source in a serum-free protein-free cell culture medium HyQ PF-CHO. The same experiment repeated using a higher seeding cell density of 1.0 × 106 cells/ml using CHO-K1 cells, CHO-DG44 cells and HEK293 cells in DMEM/F12-based Protein-Free Chemically Defined Media (PFCDM). HyQ PF-CHO MPS and PFCDM media are commercial products. Their formulation is proprietary. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5372174/pdf/srep45216.pdf |
|
Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |