Cell line/type | CHO (Chinese Hamster Ovary) CHO-K1 |
---|---|
Species | Hamster |
Animal free | Yes |
Product | PFCDM medium |
Leong, D. S. Z., Teo, B. K. H., Tan, J. G. L., Kamari, H., Yang, Y. S., Zhang, P., & Ng, S. K. (2018). Application of maltose as energy source in protein-free CHO-K1 culture to improve the production of recombinant monoclonal antibody. Scientific reports, 8(1), 4037. In this study, to characterize the kinetics of maltose metabolism, SH87, a CHO-K1 cell line that produces an anti-Her2 monoclonal antibody13 was cultured in a protein free chemically defined medium (PFCDM) containing 4 g/l glucose supplemented with 0.5, 1, 2, 3, 10 or 20 g/l maltose, which gave maximum viable cell densities greater than 8 × 106 cells/ml. The precise formulation of PFCDM is patented. https://patents.google.com/patent/WO2017146646A1/en https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5840386/pdf/41598_2018_Article_22490.pdf |
|
Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Unspecified | Unspecified |