Cell line/type | CHO (Chinese Hamster Ovary) CHO-K1 |
---|---|
Species | Hamster |
Animal free | Yes |
Product | Modified CD CHO Bort et al. 2010 |
Bort, J. A. H., Stern, B., & Borth, N. (2010). CHO‐K1 host cells adapted to growth in glutamine‐free medium by FACS‐assisted evolution. Biotechnology journal, 5(10), 1090-1097. In this study, the CHO-K1 cell line was adapted to a chemically defined medium (CD CHO medium) and suspension growth within 3 weeks. Subsequently, the glutamine concentration was stepwise reduced from 8 to 4 and 2 mM. After each reduction, both the final cell concentration in the batch and the viability decreased. To force a rapid evolution of cells to achieve high final cell concentrations, cells were seeded at high densities (10(7) cells/mL) and surviving cells were sorted by FACS or MACS when viability declined to 10% (typically after 24 h). Sorted cells were grown in batch until viability declined to 10% and viable cells recovered again. The final sorted population was able to reach comparable or even better viable cell concentrations and showed a significantly improved viability compared to their ancestors. The 2 mM glutamine-adapted cell line was directly transferred into glutamine-free medium and was able to grow at comparable rates without requiring further adaptation. Cells compensated the lack of glutamine by increasing their consumption of glutamate and aspartate. CD CHO medium is a commercial product. The formulation is proprietary. https://onlinelibrary.wiley.com/doi/pdf/10.1002/biot.201000095 |
|
Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |
Contains phenol red > No | No |
Antibiotics free > Yes | Yes |