Cell line/type | Whole embryo |
---|---|
Species | Mouse |
Animal free | Yes |
Product | SF-VCM-SPS |
Lee, J. S., Kim, J. H., Seo, Y. S., Yang, J. B., Kim, Y. I., Kim, H. J., & Lee, K. H. (2013). The influence of serum substituents on serum-free Vero cell conditioned culture media manufactured from Dulbecco's modified Eagle medium in mouse embryo culture. Obstetrics & gynecology science, 56(5), 320-329. This study was conducted to examine the influences of supplementation of the serum substituents and available period of serum-free Vero cell conditioned media (SF-VCM) manufactured from Dulbecco's modified Eagle medium cultured with Vero cells for in vitro development of mouse preimplantation embryos. The rate of embryonic development after 96 hours (hatching blastocyst</=) was significantly higher in SF-VCM supplemented with 10% SPS. The formulation of SF-VCM is listed in the Materials and Methods, under the heading; 2. Preparation of culture media, and Table 1. Compositions of DMEM-G¼ GP used in this experiment. The most effective type and the most potent concentration of serum substituents added to SFVCM are listed in Table 3. & Table 4. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3784127/pdf/ogs-56-320.pdf |
|
Source | Literature - own formulation |
Chemically defined > No | No |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |