Cell line/type | Whole embryo |
---|---|
Species | Mouse |
Animal free | No |
Product | KnockOut DMEM Kalasar et al. 2014 |
Kalaskar, V. K., & Lauderdale, J. D. (2014). Mouse embryonic development in a serum-free whole embryo culture system. Journal of visualized experiments: JoVE, (85). In this study, was shown that mouse embryos, when cultured in a defined medium in an atmosphere of 95% O2 / 5% CO2 in a rolling bottle culture apparatus at 37 degrees C for 16-40 hr, exhibit morphological growth, and development comparable to the embryos developing in utero. The authors believe this method will be useful for investigators needing to utilize whole embryo culture to study signaling interactions important in embryonic organogenesis. A detailed description of the media preparation and formulation is listed in the Protocol, under the heading; 1. Preparation of Culture Media. KnockOut Serum Replacement (KSR) is a proprietary commercial product. https://www.jove.com/pdf/50803/jove-protocol-50803-mouse-embryonic-development-serum-free-whole-embryo-culture |
|
Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |