Cell line/type | Whole embryo |
---|---|
Species | Mouse |
Animal free | Yes |
Product | N2B27 Drakou et al. 2015 |
Drakou, K., & Georgiades, P. (2015). A serum-free and defined medium for the culture of mammalian postimplantation embryos. Biochemical and biophysical research communications, 468(4), 813-819. In this study N2B27, a serum-free and defined medium was investigated whether the medium can support the in vitro development of postimplantation mammalian embryos. N2B27 medium allowed pre-gastrulation mouse embryos isolated at embryonic day 5.5 to develop to advanced gastrulation, reaching the mid- and late primitive streak stages. The formulation of N2B27 can be found online--> http://cshprotocols.cshlp.org/content/2017/5/pdb.rec096131.full?text_only=true. https://www.sciencedirect.com/science/article/pii/S0006291X15309074 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Antibiotics free > Yes | Yes |
Contains phenol red > No | No |