Cell line/type | Whole embryo |
---|---|
Species | Bovine (cow) |
Animal free | Yes |
Product | Modified CJ1 (cryopreservation) |
Lim, K. T., Jang, G., Ko, K. H., Lee, W. W., Park, H. J., Kim, J. J., ... & Lee, B. C. (2008). Improved cryopreservation of bovine preimplantation embryos cultured in chemically defined medium. Animal reproduction science, 103(3-4), 239-248. DOI: 10.1016/j.anireprosci.2006.12.020 The aim of this study was to examine the effects of modifications to a standard slow freezing protocol on the viability of in vitro produced bovine embryos. Bovine oocytes were matured, fertilized with frozen-thawed semen, and presumptive zygotes cultured in defined two-step culture media. The standard freezing medium was 1.5M ethylene glycol (EG), 0.1M sucrose, 10% fetal bovine serum (FBS) in Dulbecco's phosphate buffered saline (D-PBS). In conclusion, this study showed that modifying the cryopreservation method for bovine embryos by adding 0.1M sucrose and 0.4% Albumax® I and replacing sodium chloride with choline chloride enhanced the survival of embryos in vitro and allows post-thaw embryos to more efficiently develop into term calves. The modifications of CJ1 medium are specified in the results. The formulation of CJ1 is listed in a previous article, Stachecki et al. 1998. |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |