Cell line/type | Whole embryo |
---|---|
Species | Bovine (cow) |
Animal free | Yes |
Product | Dulbecco’s phosphate buffered saline-sericin (cryopreservation) |
Isobe, T., Ikebata, Y., Onitsuka, T., Do, L. T. K., Sato, Y., Taniguchi, M., & Otoi, T. (2013). Cryopreservation for bovine embryos in serum-free freezing medium containing silk protein sericin. Cryobiology, 67(2), 184-187. In this study, the effects of sericin supplementation for serum-free freezing medium was examined on the survival and development of bovine embryos after freezing–thawing and direct transfer to recipients. When in vitro-produced bovine embryos were frozen conventionally in the freezing medium supplemented with various concentrations (0.1%, 0.5%, and 1.0%) of sericin, the percentages of damaged zona pellucida, survival, and development of frozen-thawed embryos were similar to those of embryos frozen in freezing medium supplemented with 0.4% BSA and 20% FBS. The formulation of the serum-free, Dulbecco’s phosphate buffered saline + sericin based medium is listed in the Materials and Methods, under the heading; Experimental design. |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > No | No |
Antibiotics free > No | No |