Cell line/type | Whole embryo |
---|---|
Species | Bovine (cow) |
Animal free | No |
Product | CDM-PES |
Barceló‐Fimbres, M., & Seidel Jr, G. E. (2007). Effects of fetal calf serum, phenazine ethosulfate and either glucose or fructose during in vitro culture of bovine embryos on embryonic development after cryopreservation. Molecular Reproduction and Development: Incorporating Gamete Research, 74(11), 1395-1405. This study investigated effects of hexoses, fetal calf serum (FCS), and phenazine ethosulfate (PES) during the culture of bovine embryos on blastocyst development and survival after cryopre- servation by slow freezing or vitrification. The basal, control medium was chemically defined (CDM) plus 0.5% fatty acid-free BSA. No differences were found for additives (P > 0.1) control, FCS, or PES for blastocysts per oocyte or per 8-cell embryo. Culture of embryos with PES, which reduces cytoplasmic lipid content, improved cryotolerance of bovine embryos. The formulation of the control medium is previously listed in De La Torre-Sanchez et al., 2006b. https://www.ncbi.nlm.nih.gov/pubmed/17342731 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |